I have a feeling that many more people have experience with protein expression (even GFP) than protein purification, could people maybe post resources/guides that explain the process more thoroughly? Something like the review on e.coli expression from the open bio-economy ENZYME EXPRESSION GUIDE? Or any other guide? Something wide, and maybe also something more specific to His tagged proteins?
There has also been a lot of discussion about the nickel columns and getting them, could somebody advise which ones are the best to buy?
Thanks a lot