Hi All
Anna Bird who worked with Open Bioeconomy Lab during her PhD has recently published a paper (with her primary supervisor Prof Lisa Hall) on using flow reactors to turn genomic DNA into dNTPs, following on from her previous paper exploring the biochemistry and enzymes needed for this process.
Bird, Anna R., and Elizabeth AH Hall. “Active diagnostic ingredients (ADIs) for PCR: A mini‐bioreactor producing dNTPs with silica immobilized R5‐kinases.” Biotechnology and Bioengineering (2024).
https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/bit.28837
A self-immobilizing R5-silaffin kinase fusion enzyme was designed for immobilization on silica, converting dNMPs to their respective dNTP ADIs for PCR in a R5-kinase mini-bioreactor, designed to be implemented in a reusable device, stable over 2 months, when stored at 4°C.
dNTPs are one of the reagents that are still typically shipped via cold chain and we don’t know of any manufacturing facilities in Africa or Latin America. If you do, let us know! Local manufacturing alongside PCR enzymes could be really useful. One other thing I couldn’t find data for is the stability of dNTPs during shipping. Solis Biodyne says storing for one month at RT should be fine.
This expired patent from Roche suggests that you can store purified dNTPs for 200+ days at 20° C or 90 days at 35° C at a higher pH like 8.3. Maybe we should try this between some Reclone nodes? 
Hence it has turned out that at increased pH values no by-products are formed at all which could impair the use of the dNTPs e.g. for the PCR reaction. Even after ca. 90 days at a temperature of 35° C. the PCR function test is positive.
Jenny