Bi-weekly reclone meeting starting this Wed (Feb 3) 4 pm GMT

Community Projects
#1

Hi All,

Looks like next week Wed (Feb 3rd) 4 pm GMT would work best for all of us for our first meeting. There will be following meetings every two weeks, same time!

Calendar invite for meetings: https://calendar.google.com/calendar/u/0?cid=Y3VpZzk3YjdycWpmcG1jdWZkcDg0M3IwdGdAZ3JvdXAuY2FsZW5kYXIuZ29vZ2xlLmNvbQ

The meeting will be recorded and an update will be posted on the forum! But it would always be nice to have people participating in planning and discussions.

#2

Thanks everyone for joining us! Here are the quick meeting notes from yesterday:
Reclone Meeting 3rd February

The Inaugural Reclone Meeting!
Attending: Aish, Jenny, Lisa, Fernan, Chiara, Stephane, Kayla

Kick off:

  • Introduction of meeting (why/when), intro of attending members, first of fortnightly calls

  • Potential Symposia planned for end of Feb: Allow discussion and update of projects

  • Creating user profiles for project updates, what tools we are using, facilitate discussion of tools and protocols

  • Project Updates from Users

  • What we need from Reclone:
    o Platforms to log tools, reagents, and experiments. Protocols on protocols.io and discussion on forum.
    o How to collect data & information from people—survey stats (similar to google docs, intended for data distribution), what data to collect! (as a function of enzyme), testing enzyme yield & how to prioritize enzymes
    o 4-5 enzymes as a model for standards that we want people to use
    o System of incentives to get people to commit time (reward based community system), strategy to show how these enzymes/plasmids will be useful
    o Free genes—blue flame, easy access to materials and protocol (igen model)

  • Aim: market meeting more to get more attendees (target a few groups), potential of breakout rooms for further discussion, flash presentations in future meetings (few slides & results)

Actions:

  • Open thread in forum regarding assembly protocols (Fernan & Chiara)

  • Chiara to share form of OEC reagents

  • Make a prioritized list of enzymes (Klenow, Bst-LF, MMLV-RT, OpenVent, Pfu and Phusion) for testing

  • Be explicit that collection can grow if people are willing to share
    o Topic on forum about how to design and share your DNA parts

  • How to feedback on parts/tools/reagents

  • How to distribute collection better (maybe not ship in glycerol)

We look forward to seeing everyone at our next meeting on 17th February!

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