Bst Large Fragment

Bst Large Fragment

Reagent Sharers

Alex Brown

Summary

This enzyme comes in a pET15b plasmid. Information shared by Alex: This plasmid contains a DNA Polymerase which retains 5´ → 3´ polymerase activity from full length Bst DNA Polymerase, while lacking 5´ →3´ exonuclease activity. The polymerase has been described for use in applications requiring thermophilic strand displacement. Used for: Loop-Mediated Isothermal Amplification (LAMP), DNA sequencing through high GC regions, rapid sequencing from nanogram amounts of DNA template.

This enzyme seems to be codon-optimized (according to a comparison to Andy Ellington´s Bst LF from the Cellular Reagent paper; edit me if this is wrong)

Expression/Production Information (if applicable)

Protocol Information

We will publish soon our version of the expression/purification protocol at ReClone
group in protocols.io

How to get the plasmid/material/reagent

Currently in Alex´s hands, FreeGenes and Chile/Argentina
This is vector received from Alex and being used in Chile.

Terms

Under openMTA from Alex, being able to redistribute under openMTA from iBio/Chile too.

References

(PMID: 10871386, 25652028, 1793578)

Hi everyone! Is this polymerase available for commercial use?

Yes! It is distributed under the OpenMTA which explicitly allows commercial use. The Bst-LF US patent expired in 2016 so it is in the public domain.
Jenny

1 Like

Javier, do you have a protocol to share in protocols.io for BstLF?
(all protocols here: https://www.protocols.io/groups/recloneorg-the-reagent-collaboration-network/publications)

I hope to have one by the end of the week. Based on Alex Brown’s protocol