Bst Large Fragment
This enzyme comes in a pET15b plasmid. Information shared by Alex: This plasmid contains a DNA Polymerase which retains 5´ → 3´ polymerase activity from full length Bst DNA Polymerase, while lacking 5´ →3´ exonuclease activity. The polymerase has been described for use in applications requiring thermophilic strand displacement. Used for: Loop-Mediated Isothermal Amplification (LAMP), DNA sequencing through high GC regions, rapid sequencing from nanogram amounts of DNA template.
This enzyme seems to be codon-optimized (according to a comparison to Andy Ellington´s Bst LF from the Cellular Reagent paper; edit me if this is wrong)
Expression/Production Information (if applicable)
We will publish soon our version of the expression/purification protocol at ReClone
group in protocols.io
How to get the plasmid/material/reagent
Currently in Alex´s hands, FreeGenes and Chile/Argentina
This is vector received from Alex and being used in Chile.
Under openMTA from Alex, being able to redistribute under openMTA from iBio/Chile too.