Good afternoon everyone!
We are trying to produce at CRI both Bst-LF and M-mmlv proteins. The results on the SDS page seems a bit weird. We wanted to upload them so you may help us troubleshooting what’s going on, and hopefully that could serve for solving doubst of future people trying this protocol.
SDS page results
m-mlv questions / problems
Are we losing protein in the NiNTA flowthrough or is other 60kDa proteins?
It seems that we are loosing protein in the NiNTA wash right?
Should we incubate ON with DTT for removing the sulfur corsslinked species?
Are we loosing protein in the flowtrough or they are other 60kDa e.coli proteins?
Thank you in advance!