DpnI expression from Open Enzyme Collection?

Open DNA Collections
1

Hi All

Has anyone had success constructing and expressing DpnI yet?

Jenny

2

Hi Jenny,

I did with using JM110 as bacterial strain and tac promoter in the plasmid. Since there is no JM110(DE3) (need a pricey lysogenization kit for this…) you can’t use regular T7/lac system. The yield is not much but not abysmal.

Cihan

3

Hi Chihan,
Thank you for your suggestion! We’ll give this a try as well. Could you please share the DpnI expression and purification protocol you used in JM110 cells? Additionally, if possible, could you provide the sequence of the tac promoter you used? If I understand correctly, we can simply insert the tac promoter upstream of the ORF in Open enzyme plasmid, and that should work, right?