DpnI expression from Open Enzyme Collection?

Open DNA Collections
1

Hi All

Has anyone had success constructing and expressing DpnI yet?

Jenny

2

Hi Jenny,

I did with using JM110 as bacterial strain and tac promoter in the plasmid. Since there is no JM110(DE3) (need a pricey lysogenization kit for this…) you can’t use regular T7/lac system. The yield is not much but not abysmal.

Cihan