Enzyme Purification Meeting

Open DNA Collections Enzyme Expression Protocols
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Hey folks!

In the JOGL weekly it came up to organize an enzyme purification meeting, to discuss protocols and share experiences/ask questions/inspire each other :).

If you are interested, here is a dudle:
https://dudle.inf.tu-dresden.de/enzpur/

The time is in CEST, so make sure to use https://www.thetimezoneconverter.com/

See you soon & take care!
Kathrin

1 Like
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Hey again,
We meet tomorrow, Friday at 7pm CEST (12 pm CST, 10 am PDT/MST)
The meeting room is here: https://meet.inheaden.io/b/kat-hel-1v3

Agenda will be as follows:

  1. Status report of whoever wants to
  2. Discussion of purification protocols on protocols.io -> Bst & MMLV
  3. Resources/Literature Share and Discussion
  4. Discussion on IT-infrastructure

Hope to see you soon :slight_smile:
Kathrin

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You can also send me questions in advance, if you can‘t make it & we try to discuss them & get back to you: kathrin.hadasch@lab3.org

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Hi everyone, I found this short paper [http://dx.doi.org/10.5483/bmbrep.2008.41.2.108]
Which basically says e.coli only has like 17 heat-stable proteins, most of which are involved in folding and such, and as we think these enzymes should work pretty good even without any purification i.e. “Cellular reagents”, seems to me we could get away with only a thermal precipitation? Has anyone tried this or heard about it or knows how to do it? :smiley:
Or maybe at least add it as a step before the Ni Chromatography?

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Hey guys!
Thank you all for joining in! I hope it helped a little.
Here are the notes with the links from the chat: https://drive.google.com/file/d/14QgDlFLq0N9MjXx8zpk08TAX46lLWNMJ/view
The recording is here: https://youtu.be/QkmzpsHVegM
Keep up the good spirit & see you at the reclone symposium on July 29th :slight_smile:
Kathrin

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The google drive was not shared, here you have a public link: https://drive.google.com/file/d/14QgDlFLq0N9MjXx8zpk08TAX46lLWNMJ/view?usp=sharing