Enzyme Purification Meeting

Open DNA Collections Enzyme Expression Protocols
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Hey folks!

In the JOGL weekly it came up to organize an enzyme purification meeting, to discuss protocols and share experiences/ask questions/inspire each other :).

If you are interested, here is a dudle:
https://dudle.inf.tu-dresden.de/enzpur/

The time is in CEST, so make sure to use https://www.thetimezoneconverter.com/

See you soon & take care!
Kathrin

1 Like
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Hey again,
We meet tomorrow, Friday at 7pm CEST (12 pm CST, 10 am PDT/MST)
The meeting room is here: https://meet.inheaden.io/b/kat-hel-1v3

Agenda will be as follows:

  1. Status report of whoever wants to
  2. Discussion of purification protocols on protocols.io -> Bst & MMLV
  3. Resources/Literature Share and Discussion
  4. Discussion on IT-infrastructure

Hope to see you soon :slight_smile:
Kathrin

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You can also send me questions in advance, if you canā€˜t make it & we try to discuss them & get back to you: kathrin.hadasch@lab3.org

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Hi everyone, I found this short paper [http://dx.doi.org/10.5483/bmbrep.2008.41.2.108]
Which basically says e.coli only has like 17 heat-stable proteins, most of which are involved in folding and such, and as we think these enzymes should work pretty good even without any purification i.e. ā€œCellular reagentsā€, seems to me we could get away with only a thermal precipitation? Has anyone tried this or heard about it or knows how to do it? :smiley:
Or maybe at least add it as a step before the Ni Chromatography?

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Hey guys!
Thank you all for joining in! I hope it helped a little.
Here are the notes with the links from the chat: https://drive.google.com/file/d/14QgDlFLq0N9MjXx8zpk08TAX46lLWNMJ/view
The recording is here: https://youtu.be/QkmzpsHVegM
Keep up the good spirit & see you at the reclone symposium on July 29th :slight_smile:
Kathrin

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The google drive was not shared, here you have a public link: https://drive.google.com/file/d/14QgDlFLq0N9MjXx8zpk08TAX46lLWNMJ/view?usp=sharing