This protocol describes a simple, cost-effective method for purifying Green Fluorescent Protein (GFP) from E. coli using a sucrose gradient. It avoids toxic chemicals and expensive chromatography systems, making it accessible and environmentally friendly. The method preserves GFP’s fluorescence and is suitable for applications in biomedical imaging, molecular tagging, and gene expression education laboratories.
with further improvements, it could be used in research studies.