Open Source / Free Use E. coli Expression Strain

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Screenshot 2025-05-23 142652
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Hi everyone,

I’m excited to share that our new open-source, free-use E. coli expression strain developed through the OpenBioMAPS project has successfully produced mCherry fusion proteins. Preliminary benchmarking against BL21(DE3) indicates that this strain may yield higher amounts of protein after overnight induction with IPTG.

Looking forward to further testing and refining its performance. Feedback and collaboration are very welcome!

Thanks,

Aaron

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Dear Aaron,

This is great work. I would like to get some of the opensource E coli. Could you point me in how might be able to get some cell. Thank you so much. You can also reach me at (erosca@ashesi.edu.gh).
Best wishes,
Elena

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Hi Aaron,

This is fantastic news! An open-source, high-performance E. coli expression strain could be a game-changer for the community. I’d love to learn more about your work—would you be able to share some additional details?

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Hi Elena,

After full genomic sequencing, we plan to deposit the strains in Addgene with OpenMTA in bulk by the end of the year! We’ll keep you posted! :slight_smile:

Kind regards,
Aaron

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Hi Ahmed,

I’ll be sharing some infographics about this soon. Basically, I used Lambda Red recombination which is now outside of patent restrictions to insert the T7 RNA polymerase gene from the Reclone DNA collection into the Lac operon of E. coli MG1655. :slight_smile: