OpenVent (DNA Polymerase from, sold as DeepVent by NEB)
Open Bioeconomy Lab/Free Genes/Alex Brown
OpenVent is an off-patent version of a thermostable archaeal family-B DNA polymerase from Pyrococcus strain GB-D which is sold under the trademark DeepVent by NEB.
It has 5X higher fidelity than Taq and extremely high thermostability (half-life of 23 hours at 95°C). It is ideal for GC-rich, looped sequence and other difficult templates.
OpenVent is codon-optimised for expression in E.coli and is very robust and amenable to most forms of expression and purification. Open Bioeconomy Lab have expressed it as cellular reagents very effectively.
It can usually be expressed in the same way as Taq polymerase. Options can be found in this folder.
Protocols are available at NEB.
How to get the plasmid
Contact mail+reagents at mail.reclone.org with your shipping address and courier account number.
If you would like the CDS as a DNA part in a cloning vector, it is part of the Open Enzyme Collection Part 1 available from Free Genes.
All materials will be distributed under an OpenMTA meaning they can be used for commercial purposes and be redistributed. In advance of receiving the materials, you should ask your institution to sign the OpenMTA Master Agreement.
Reagents you have produced using this kit are intended for research use only. In order to use them for clinical diagnostics you must follow all local regulatory processes.
The following terms of the OpenMTA also apply:
No Implied License: Except for the rights expressly granted herein, the Recipient agrees that no other rights or licenses, whether express or implied, are granted to the Recipient under any patent, patent application, or other proprietary right of the Provider. As between the parties, each retains all right, title, and interest in works and inventions made by its personnel, and nothing herein shall be construed to transfer ownership of any invention, patent, patent application, or other proprietary right.
Liability: Except to the extent prohibited by law, the Recipient assumes all liability for damages that may arise from the use, storage or disposal of the Material. The Provider will not be liable to the Recipient for any loss, claim or demand made by the Recipient, or made against the Recipient by any other party, due to or arising from the use of the Material by the Recipient, except to the extent permitted by law when caused by the gross negligence or willful misconduct of the Provider.
No Warranties: Any Material delivered pursuant to the Agreement is understood to be experimental in nature and may have hazardous properties. THE PROVIDER MAKES NO REPRESENTATIONS AND EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED. THERE ARE NO EXPRESS OR IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR THAT THE USE OF THE MATERIAL WILL NOT INFRINGE ANY PATENT, COPYRIGHT, TRADEMARK, OR OTHER PROPRIETARY RIGHTS.