OpenVent-mCherry is working in Chile (Latam)

Open DNA Collections Enzyme Expression Protocols
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Dear all,

This is a post to say that we have been implementing the cellular reagent version of the OpenVent_mCherry in Santiago (Chile) and it’s working! A big shoutout to our collaborators in Mendoza (Argentine) from Dra. Damiani’s lab (Mariangeles Avila in particular) who facilitated us the plasmid for OpenVent_mCherry.

We did have some difficulty with the induction of the expression of the polymerase though, so we wanted to share the experience. Here’s a document that systematizes what we have been doing, hopefully someone finds it useful.

Thanks to @jenny_molloy too for her guidance during her stay in Santiago a couple of months ago. We have a workshop in a couple of weeks with high school teachers in Southern Chile to produce the OpenVent locally so we are pretty excited about those results!

All the best,
Séverine.

4 Likes
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Hi Severine,

We would be very interested to try that in our lab. Any chanced you could share?

Cheers,
Adrian

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Hi Adrian! Sure, where is your lab located? The only issue is that we don’t have money for shipments :face_with_peeking_eye:

Let me know :wink: