OpenVent purification

Open DNA Collections Enzyme Expression Protocols
1

Hi all.

We are producing OpenVent-mCherry at my lab and we would like to try an AKTA purification instead of the classic Bench purification. Does someone have tried this? In that case, would you be able to share the protocol?

Thanks!

Pablo

2

Hi Pablo!

We don’t have OpenVent-mCherry but we purify diferent chromoproteins (fuGFP, aeBlue, Yukon, Dasher…) by AIEX in an AKTA Go. We follow this procedure. We adapted the flow rate and CV to our system. If you want I can share you a gradient and a step elution chromatogram so you know what to expect

3

Hi Jon.
We attempt to purify the protein by IMAC, however, I´ll take a look to the procedure you´ve provided. Thank you very much!

Pablo