Status Update: In Production
The “ready to express” plasmids (first rows in this spreadsheet) have been sent to Twist and as per their contact:
“Twist is prioritizing COVID-19 related projects, so I’ll flag this order and it will be pushed to the top of our synthesis queue.”
We hope this means the shipment will arrive in a few weeks to Stanford and then out as soon as possible to you all. More news as we have it…
Research in Diagnostics Toolkit
The Reclone/org network, also Free Genes and we will deposit in Addgene.
The toolkit is a combined effort of the Open Bioeconomy Lab at University of Cambridge (Jenny Molloy, Chiara Gandini), the Federici Lab at Pontificia Universidad Católica de Chile (Fernan Federici, Isaac Núñez, Tamara Matute, Anibal Arce), the Free Genes Project (Drew Endy, Keoni Gandall, Isaac Larkin) and Open Science Network Society (Scott Pownall). We are also grateful for the support of Ginkgo Bioworks.
The Research in Diagnostics Toolkit is a set of ready-to express vectors and (optionally) DNA parts for the most common molecular diagnostic techniques.
Expression plasmids that are IPTG/Lactose inducible with a 6xHis-Tag for:
- HIV reverse transcriptase
- OpenVent (DeepVent) DNA polymerase
- OpenScript (SuperScriptII) reverse transcriptase
- gp32 (ssDNA binding protein)
- Bsu (strand dsplacing polymerase)
- UvsY (T4 cofactor)
- UvsX (RCA-like recombination protein)
- Murine RNA inhibitor
- RNaseH from Tth
- RNaseH from Escherichia coli
- T7 RNA polymerase
- TEV protease
Other useful enzymes
- PBCV-1 ligase
- Horse radish peroxidase
Expression/Production Information (if applicable)
We do not have fully validated expression protocols but there are a lot out there in the literature which you can see summarised on the Enzyme Expression Guides page at Open Bioeconomy Lab.
The reagents can be used in most protocols for PCR, RT-(q)PCR, RT-LAMP, RPA, RCA and a variety of other molecular research techniques.
How to get the plasmid/material/reagent
Note that the collection is not yet synthesised but you can sign up for delivery here
If you have any queries, contact mail+reagents at mail.reclone.org
All materials will be distributed under an OpenMTA meaning they can be used for commercial purposes and be redistributed. In advance of receiving the materials, you should ask your institution to sign the OpenMTA Master Agreement.
Reagents you have produced using this kit are intended for research use only. In order to use them for clinical diagnostics you must follow all local regulatory processes.
The following terms of the OpenMTA also apply:
No Implied License: Except for the rights expressly granted herein, the Recipient agrees that no other rights or licenses, whether express or implied, are granted to the Recipient under any patent, patent application, or other proprietary right of the Provider. As between the parties, each retains all right, title, and interest in works and inventions made by its personnel, and nothing herein shall be construed to transfer ownership of any invention, patent, patent application, or other proprietary right.
Liability: Except to the extent prohibited by law, the Recipient assumes all liability for damages that may arise from the use, storage or disposal of the Material. The Provider will not be liable to the Recipient for any loss, claim or demand made by the Recipient, or made against the Recipient by any other party, due to or arising from the use of the Material by the Recipient, except to the extent permitted by law when caused by the gross negligence or willful misconduct of the Provider.
No Warranties: Any Material delivered pursuant to the Agreement is understood to be experimental in nature and may have hazardous properties. THE PROVIDER MAKES NO REPRESENTATIONS AND EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED. THERE ARE NO EXPRESS OR IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR THAT THE USE OF THE MATERIAL WILL NOT INFRINGE ANY PATENT, COPYRIGHT, TRADEMARK, OR OTHER PROPRIETARY RIGHTS.