RNAse expression: experience and working protocol

Hello everyone,

Our lab is interested in producing RNAse for DNA purification workflows. Does anyone have experience in expressing this particular clone (https://www.addgene.org/165565/)? We want to be sure that it is possible to express it in E. coli before acquiring the plasmid.

Looking forward to receiving some feedback.

Cheers,

Pablo

您好,RNAse无法直接使用细菌进行原核表达纯化,我建议您考虑使用革兰氏阴性菌的周质空间表达载体进行表达,