according to this research you can use the microwave instead of autoclave to sterilize culture media for microbiology, this can save you a lot of time and energy in case you are working in very! tight budget lab.
i will try it ang give feedback about it as soon as it gives results.
#tips_tricks
Microwave does not work (in my experience) with endospores and yeast spores. But a cheap alternative is to use a pressure cooker + a vegetable steamer (so that your media bottles are not touching the bottom of the pressure cooker). Fill it with 3-4 fingers of water and boil away. The ones that you can see the temperature works the best. We still use a pressure cooker in the lab when the autoclave breaks down. A good sized pressure cooker can accomodate up to 3 1L GL45-capped bottles or a 2L erlen.
Cihan
very insightful thank you dr.cihan
currently we are only using bacetria
and so far the results are quite good, and this method saved us a lot of time and effort for our routine culture, we don’t have technicians so the PhD student must do everything from scratch.
i will be happy to try the method you described and share the results later here.
best regards