Nucleus

Getting Started If you’re new to Reclone.org or need help finding your way around, this is the place to start! Have a read to find groups, categories and items of interest. Publications The Publications category is for posting about publications - your own or others - that you wish to share with the community.

Topic	Replies	Views	Activity
Introducing yourself and the work you do Nucleus welcome-calls We can use this thread to introduce each other and share the work we are all doing. your name place where you work/ research area people you work with/projects on COVID19-related projects designing/expressing/testing a…	24	2600	8 August 2025
About the Nucleus category Nucleus	0	851	27 April 2020
96-well plate-based minipreps - anyone with experience? Nucleus	0	11	6 October 2025
Low-Cost Sucrose Gradient Protocol for Green Fluorescent Protein (GFP) Purification from Recombinant E. coli: A Visual Fluorescence-Based Method for Resource-Limited Laboratories Publications	0	33	26 September 2025
New reproducibility software seeking feedback Nucleus community-collaborations	0	45	21 September 2025
Where to find the Free Genes Plate older distribution Maps? Nucleus	5	99	11 September 2025
Plate-based expression goes large! Nucleus	4	327	24 August 2025
What scripts are you using for DNA synthesis prep? (codons, domestication, syntax, RE cut sites) Nucleus	2	149	21 August 2025
Guidance on Open Enzyme Collection and IP Considerations Nucleus help-wanted	0	94	18 August 2025
Instant enzymes: Systems engineering approach to rapid low cost production of thermostable molecular biology enzymes Publications	0	93	18 August 2025
Announcing a Reclone Node at Universidade de São Paulo, Brazil 🇧🇷 Nucleus	1	182	6 August 2025
Anyone tried Vesicle Nucleating peptides (VNps)? Nucleus	2	145	3 August 2025
The Role of AI in Advancing Biotechnology: Current Applications & 58 Free AI tools & Future Trends & Implications for Global Health Equity Publications	0	126	31 July 2025
New Publication: Reducing Supply Chain Dependencies for Viral Genomic Surveillance: Get by with a Little HELP from Commercial Enzymes already in your Lab Freezer Publications	0	183	24 June 2025
New Preprint: International Multi-site Implementation of Local Cell-Free Protein Biomanufacturing to Advance Health and Research Equity Publications	1	108	30 July 2025
New Publication: Unveiling the Frontiers of Synthetic Biology in Brazil: Pioneering the National Synthetic Biology Network Publications	0	111	30 July 2025
Luminol Synthesis Protocol from Locally Available Materials Nucleus	0	156	16 July 2025
Looking for participants in short interviews to help us design better protein purification protocols & a "biomanufacturing in a box" kit Nucleus	10	260	9 July 2025
Affordable Small Molecules as Promising Fluorescent Labels for Biomolecules Publications	2	312	9 July 2025
Paper-based devices diagnose malaria in asymptomatic people during field test Nucleus	0	120	4 July 2025
DIY NanoDrop Microvolume Spectrophotometer Protocol for Low-Resource Labs Nucleus	6	312	29 June 2025
Making plant-based leather from coffee ground and yerba matte remains (from waste to leather) Nucleus	0	272	11 June 2025
Preparing an Eco-Friendly pH Indicator Using Red Cabbage Extract suitable for Low-Budget labs Nucleus	0	314	5 June 2025
Cradle.bio are funding the creation of an open-source antibody dataset Nucleus	0	197	23 May 2025
L-Arginine Hydrochloride Enables Cold-Chain-Free DNA Polymerase Storage Publications	0	190	19 May 2025
Eco-Friendly Efficient DNA Staining Using Modified Methylene Blue Solution: A Frugal and Safer Alternative to Ethidium Bromide in Low-Resource Biotechnology Laboratories Nucleus	0	320	1 May 2025
Synthetic Osmolytes for Extended Plasmid Storage at Room Temperature Publications	0	207	31 March 2025
Issues with Reproducibility in Open-Source Biotech Experiments Nucleus help-wanted	3	223	27 March 2025
Making a homemade low-cost agarose gel electrophoresis tank apparatus Nucleus	2	381	11 March 2025
Lysis - affordable alternative Publications	0	207	11 March 2025