Hello All,
Happy New Year!
You are all invited to our Reimagining Biomanufacturing Journal Club next week which will take placed on 2025-01-14T15:00:00Z→2025-01-14T16:30:00Z (Are we meeting yet?).
For this session, Jenny will be discussing the paper: “Protein purification with light via a genetically encoded azobenzene side chain” .
Why is this paper reimagining biomanufacturing? a long-standing limitation to purification of proteins by affinity chromatography is the need to elute in a specific buffer that disrupts the interaction of the affinity tag and matrix. Using light to release the protein or as these authors name it: “Excitography” (!) allows for elution directly into any buffer of choice, reducing processing steps and reagent requirements. It is also a neat demonstration of a practical use of non-natural amino acids to introduce new functionality to proteins. The peptide is only 2-4 amino acids, shorter than all recombinant affinity tags described to date. Release of the protein can be induced by cheap UV LEDs, which lends the technique to interesting practical applications, such as automated high-throughput screening campaigns in microtiter plates and even large scale matrices with embedded lights.
Join us via Zoom: Launch Meeting - Zoom
All are welcome to attend and take part.
Feel free to invite and share this to others in your networks who might be interested!
If you are interested in presenting a paper, please contact me to make arrangements.
See you all at the meeting next week!
Best wishes,
Kominist