Looking for participants in short interviews to help us design better protein purification protocols & a "biomanufacturing in a box" kit

Nucleus
1

Hi!

My research groups are looking to run some short interviews (30-45 min) with:

a) small companies or institutions manufacturing molecular biology enzymes, to get some ideas for improved expression and purification technologies (prioritising those in the global South, but we are geographically inclusive if you are willing!);

b) organisations who would be interested to have a “biomanufacturing in a box” kit for education or getting started with enzyme production for research.

If you have any ideas for invitees, or would be willing to be interviewed yourself, please let me know!

Jenny

2 Likes
2

Hello Jenny!

We have always been interested in these kind of efforts!. As you know we are based in Mexico (CDMX) and we have been recently involved with more students, academics and independent researchers in Mexico and central America due to our participation in the HTGAA course by the MIT media Lab. Count with us to expand the network!

Best,

David

David J. Castillo Ph.D.,

Glyxon Biolabs

glyxonbiolabs@gmail.com
davesnetvault@me.com

+52 (55) 70 94 13 76 (Home office)

+52 1 (55) 41 44 76 07 (Mobile)
+52 (55) 56 79 07 28 (Lab)

www.glyxon.com

3

Here at pacific coast Biologics, we manufacture a few enzymes for internal use and we can definitely help with purification.

Support@pacificcoastbio.com

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Hello David,

Thank you for your interest. I’ll be in touch shortly via email with available meeting times and further details.

Best regards,
Davida Ruharo

5

Hello,

I’ll be reaching out as well to schedule a meeting at your convenience.

6

Hello Dr. Molly,

I am interested to participate in the interview. One of the recent technologies that caught my interest is cSAT 2.0, developed by a Chinese research group. It presents a remarkably simple and promising method for protein expression. I was particularly intrigued by its potential and encouraged some colleagues in Germany to test it with their proteins of interest.

However, their results showed significantly lower yields than those reported in the original publication. I suspect this discrepancy might be due to toxicity or other context-specific factors. This suggests that the system may require further optimization and testing across different protein targets.

Given the current observations, I believe there may be an opportunity to develop a modified variant of the cSAT 2.0 technology. This could potentially address intellectual property considerations while improving its efficiency and usability

https://www.sciencedirect.com/science/article/abs/pii/S0167779924002907

They deposited one of there plasmids on addgene
231128

7

Following up on my previous reply regarding the cSAT 2.0 technology, I wanted to suggest an alternative that might improve both yield and purity in protein purification—particularly in light of the relatively modest results seen when my colleagues tested the system.

One promising method involves hydroxyapatite-based immobilized metal affinity chromatography (IMAC), which has been shown to efficiently purify (His)-tagged proteins. Hydroxyapatite is relatively easy to prepare, and when loaded with Fe(III), it has demonstrated a binding capacity and selectivity comparable to commercial Ni-NTA agarose resins. This makes it a cost-effective and practical alternative to Ni-NTA, especially in systems where protein yield or purity is suboptimal.

In fact, in the paper by Suen et al. (2004), the authors reported achieving over 95% purity of His-tagged proteins in a single step using Fe(III)-loaded hydroxyapatite under optimized conditions.

https://www.sciencedirect.com/science/article/abs/pii/S0021967304011422

I should clarify that these methods are not part of my own published work, but I had planned to test them independently. I’m sharing them here as a topic of mutual interest and potential co-evaluation.

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Hello Dr. Molly,

I am interested to participate in the interview and collaborate to bring this initiative over the finish line. There are a number of projects including cross collaborations with people that already responded to this topic that could be leveraged pretty much as is.

Thank you,
Adrian
Ottawa Bio Sci.

9

Hello,

With Yann (@YHK), we are happy to participate in the interview and to collaborate further. We started a small company in France to produce and sell enzymes, and we are always looking for more projects to take one.
We will also be happy to connect with the people who responded to this thread.
louise.lassalle@enzymethic.fr
y.dekermadec@enzymethic.fr

Thank you,
Louise

10

This is a good one.

Caroly

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Here,I suggest the use of magnetic beads,Magnetic beads purify proteins by using a surface coating that specifically binds to the target protein, such as a His-tag or an antibody. When added to a protein solution, the target protein attaches to the beads, and a magnet is used to pull the beads,and the bound protein away from the rest of the mixture. After washing to remove impurities, the purified protein is released using a suitable buffer. This technique seems to be simple, quick, and effective for isolating proteins in research or lab settings.

Caroly.