I’m interested in developing an “Open Cloning Kit” based in part on "Producing molecular biology reagents without purification” paper by Dr Molloy.
We will be starting with the enzyme and ligase and once the replication works redesign the enzyme as a one plasmid and incorporate some open devices in the protocol to offer a complete solution.
I have several people already interested in joining the project however of anybody else is interested I will be more than happy to include you.
This open kit could be used both for research and for education and I would expect the project to be developed in an iterative manner.
The paper is amazing and very broad in scope.
This will not include all the experiments in the paper but just the MoClo part.
You can reply here or email directly gmail to openbioscience/
Thank you for sharing this exciting initiative. I’m very interested in contributing to the Open Cloning Kit project.
I have access to a lab facility and some helping hands (my students) here in Egypt, and we can carry out experiments to support the development, particularly the MoClo part you mentioned. I’d be glad to collaborate and test the protocols in our setup.
Looking forward to following the progress and discussing how we can best get involved.
Thank you for sharing this opportunity. My group and I are interested in being part of the project. I am from INSIBIO-CONICET, Argentina.
Saludos cordiales,
PhD. Guillermo A. Vega Lopez
Universidad Nacional de Tucuman
Chacabuco 461, Tucuman
(CP: T4000ILI) -Argentina
Web of Science ResearcherID: M-3403-2014
ORCID: 0000-0002-2426-2844
Sounds interesting. Glad to collaborate in the project. Here in UPC (Lima, Peru), we work routinely in recombinant protein expression and purification. Additionally, we’re starting to work with open source devices.
We have an overwhelming interest in this project so far.
There is a great number of quality participants. I also have a number of people that are not yet in the Reclone sphere that are equally interested.
Several thoughts to start with:
This will probably deserve a meeting of its own so we’ll have to organize that.
It would be good to start a draft high level document.
As a project it should have an online place to collect materials, probably GitHub.
Also since this a project that might require instant communication please let me know if I should create a Slack group.
Keeping a project with so many participants running will require dividing the work based on capabilities, availability and interest.
Great initiative. Please count us in from Aysén, Chile.
We have the basic infrastructure to help build and test low-cost kits, and we have been working with OpenFlexure microscopy and other low-cost/open-source devices for education and research. This project would fit very well with what we are trying to develop here.
Dr. Jorge A. Toledo
Profesor Asociado
Departamento de Ciencias de la Salud
Dirección: Eusebio Lillo 667, Coyhaique. Chile
ORCID: ORCID