Open Cloning kit- Research and education

Nucleus
1

Hello all,

I’m interested in developing an “Open Cloning Kit” based in part on "Producing molecular biology reagents without purification” paper by Dr Molloy.
We will be starting with the enzyme and ligase and once the replication works redesign the enzyme as a one plasmid and incorporate some open devices in the protocol to offer a complete solution.
I have several people already interested in joining the project however of anybody else is interested I will be more than happy to include you.
This open kit could be used both for research and for education and I would expect the project to be developed in an iterative manner.
The paper is amazing and very broad in scope.
This will not include all the experiments in the paper but just the MoClo part.
You can reply here or email directly gmail to openbioscience/

Thank you,
Adrian
https://ottawabioscience.github.io/

1 Like
2

Here’s a reply you can use:

Hi Adrian,

Thank you for sharing this exciting initiative. I’m very interested in contributing to the Open Cloning Kit project.

I have access to a lab facility and some helping hands (my students) here in Egypt, and we can carry out experiments to support the development, particularly the MoClo part you mentioned. I’d be glad to collaborate and test the protocols in our setup.

Looking forward to following the progress and discussing how we can best get involved.

Best regards,
Atef.

3

Hi Adrian
I am interested, kindly incorporate me

4

Hello Adrian
I am interested, incorporate me please

5

Hi Adrian,

Thank you for share this opportunity, my group and I are interested in be part of the project, I am from UTC in Ecuador.

Saludos cordiales,

MSc. Johanna Valentina Arévalo Granda
Docente Ocasional
Universidad Técnica de Cotopaxi

6

Hi Adrian,

Such a wonderful initiative! We are interested, please incorporate me.

Best regards,

Elisa

7

Hi Adrian,

I am interested too. Please, incorporate me to the project

Best regards

8

Hello Adrian,

What a wonderful initiative!

I would be delighted to be involved. Please include me in this
opportunity.

Best regards,

Juliana Cardinali