Reclone Community Meeting on May 15th at 14:00 UTC ✨

Events
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Hello Reclone Community :wave:

You’re all invited to our Community Meeting next week, on 2024-05-15T14:00:00Z. We will hear from @ArielCerda and @FernanFederici on their 📰 New Publication: An Open One-Step RT-qPCR for SARS-CoV-2 detection from earlier this year.

:arrow_right: Join us via Zoom: Launch Meeting - Zoom

Feel free to invite and share news with others in your networks who might be interested :star_struck:

You can also check our previous meetings at Events – Reagent Collaboration Network or via the Reclone YouTube channel.
Likewise, you can see the Upcoming Events on the Reclone Calendar, tell us you’re Interested/Going, and add these to your own calendar.

See you all next week!

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:pushpin: Reclone Community Meeting Recap

Though it was more than a year ago, we extend our thanks once more for joining us for @ArielCerda and @FernanFederici inspiring talk! They shared valuable insights on “An Open One-Step RT-qPCR for SARS-CoV-2 Detection”.

Here are some highlights from the discussion for those who may have missed it:

:heavy_check_mark: They addressed reagent shortages during the COVID-19 pandemic in Chile by developing an open-source, probe-based one-step RT-qPCR kit using locally produced enzymes (MMLV reverse transcriptase and hot-start Taq DNA polymerase expressed in E. coli), achieving performance comparable to commercial kits.

:heavy_check_mark: They detailed enzyme purification protocols (nickel-NTA and affinity chromatography) shared openly, with validation showing a limit of detection of 10 copies per reaction and strong results on clinical samples (95% sensitivity, 100% specificity).

:heavy_check_mark: The method enables cost-effective, decentralised production in resource-limited settings, with extensions to dye-based alternatives, applications for other pathogens (e.g., waterborne and agricultural), educational tools, and community collaboration via platforms like Reclone.

You can check the complete session on our YouTube: [Link]

And reach out to @ArielCerda or @FernanFederici to find out more :star_struck: