hello everyone
i used this protocol (very simple) to substitute IPTG that costs 5,000,000 s.p for 1 gram to use skimmed milk which costs 16,000 s.p for litre, too induce bacteria that express GFP and has a Lac operon in , the evaluation is visual because we don’t have a spectro in our lab, but mainly the results seem promising.
i hope this helps other researchers that are trying to have frugal alternatives and express recombinant proteins with close to zero budget.
link to protocol:
Greetings Joseph,
You can also substitute lactose with skimmed milk in Studier’s autoinduction media recipe, it simply works!
Cihan
i was literally going to try that later this month,
dr.jenny suggested an auto induction media for a recombinant protein expression
and i’m trying to mae an ultra low-cost version of auto induction media.
thank you for the insight, it is very helpful to know it works beforehand.
all the best
Interesting hack! Which promoter sequence did you use?
it should work on any lacZ promoters
