hello everyone
i used this protocol (very simple) to substitute IPTG that costs 5,000,000 s.p for 1 gram to use skimmed milk which costs 16,000 s.p for litre, too induce bacteria that express GFP and has a Lac operon in , the evaluation is visual because we don’t have a spectro in our lab, but mainly the results seem promising.
i hope this helps other researchers that are trying to have frugal alternatives and express recombinant proteins with close to zero budget.
link to protocol:
