Using Skimmed milk as a low-cost alternative to IPTG in plasmid expression induction (LacZ Promoter)

hello everyone
i used this protocol (very simple) to substitute IPTG that costs 5,000,000 s.p for 1 gram to use skimmed milk which costs 16,000 s.p for litre, too induce bacteria that express GFP and has a Lac operon in , the evaluation is visual because we don’t have a spectro in our lab, but mainly the results seem promising.
i hope this helps other researchers that are trying to have frugal alternatives and express recombinant proteins with close to zero budget.

link to protocol:

dx.doi.org/10.17504/protocols.io.j8nlk81z6l5r/v1

Greetings Joseph,

You can also substitute lactose with skimmed milk in Studier’s autoinduction media recipe, it simply works!

Cihan

i was literally going to try that later this month,
dr.jenny suggested an auto induction media for a recombinant protein expression
and i’m trying to mae an ultra low-cost version of auto induction media.
thank you for the insight, it is very helpful to know it works beforehand.

all the best