Hi All
For distributing the open DNA collections, we could do with a 96-well format for plasmid minipreps.
My lab has used magnead prep on the OT2/other automation, and this was working but right now we don’t have someone experienced with the protocol and the yield wasn’t great for the time it took.
I found this protocol in an application note from Corning, using glass fiber plates that would yield 4ug so maybe 8-16 replicate plates depending on how much DNA we want to include.
Glass fiber filter plates are expensive but nowhere near as expensive as the 96-well miniprep kits available commercially. We have a plate vacuum manifold in the lab, so we’re set in that respect.
What do you think? I’m very open to other suggestions!
CSHL protocol for similar purpose: Sign In
Another related protocol from the literature: https://academic.oup.com/nar/article/25/6/1315/1203337?login=true
Example with automation: Automation Protocol for Plasmid DNA Extraction from E. coli
Jenny