We have tried multiple transformations and cannot get PCR confirmation of an appropriate insert using the M13 primers for pOpen.
The ORFs are supposed to code for ETSSB and T4 exonuclease V, respectively.
Has anyone else experienced this? Any suggestions?
Thanks for raising this @EJorgensen.
I’m looping in @fxbuson in case he has the time to test this as well, or if has already tested these sequences before.
Hi Ellen, I have not tried to transform these from the Freegenes distribution (we have the older glycerol stock-based distributions), but maybe you could try getting these parts from a different plate? The Open Enzyme Collection is also on the iGEM 2022 and 2023 distributions if I remember correctly. Here is a link to their airtable.