Hi All,
Your help needed!
We’re currently looking into creating a mini starter toolkit of parts from the Open DNA Collections (ODC) for distribution amongst the community as a ready-to-express toolkit. Example use cases could be as a kick starter for basic molecular biology research, or as an education/training resource.
What would you put in such a toolkit?
What we want to achieve?
The aim is to put the 8 most commonly used/requested parts currently in the ODC into a ready-to-express plasmid (i.e. pTI - see thread and additional info). This would then be replicated, shared amongst the Hubs, and will be made available for distribution.
If there is a lot of interest, we may consider the top 16 requested parts to include in the starter kit.
What is there currently?
The only ready-to-express toolkit we have right now is the Research in Diagnostics Toolkit (RiDT), though there are others* who are trying to do the same with the Open Enzyme Collection (OEC).
There have already been suggestions for the following parts:
- [ODC_0224] PCR (OpenVent) - already ready-to-express,
- [ODC_0219] Hi-Fi PCR (Pfu-sso7d) - already ready-to-express,
- [ODC_0223] RT (MMLV SuperScript III equivalent) - already ready-to-express,
- [ODC_0222] HIV-RT - already ready-to-express,
- [ODC_0234] LAMP (Bst-LF) - already ready-to-express,
- [ODC_0047] T7 RNA polymerase,
- [ODC_0025] T4-DNA ligase
But do let us know if you agree/disagree with these, and what else you’d want!
*@EJorgensen - I hope this is going well? Would love to hear your updates?
The ask from you?
Look through the following collections and select what you think should go into the starter toolkit:
- Open DNA Collections Phase 1 parts that are currently in distribution,
- The not yet in distribution ODC Phase 2 additions from the 2023/2024 wishlist (both the OEC extended** parts, and the yeast Protein Expression Toolkit (yPET) that @osn_scott and Ian Caven helped us develop.
Post in this thread the ones that you would like to see in the toolkit.
- Where possible, please use the ODC IDs and the part name when posting your suggestions.
**We’ll be integrating this into the main OEC and other collections in due course, but this is still a WIP. If you’d like to help with this, and/or improve the available documentation, email us at coordination@reclone.org.
Next steps
The Reclone Coordination/CZI team will review your suggestions as they come in, and collate the top suggestions to share back to the community.
We’re still working through the logistics of who, how, when we can take these ideas and make them into the starter kit, and when they might come into distribution, but we wanted to get your initial feedback at this early stage so we can make sure what is done is suited to what the community wants.
Watch this space for updates!
For now… please just post your suggestions!
Provisional deadline for first round of suggestions is 2025-02-28T23:59:00Z.
Thank you very much for your support! 